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β2 ar r11e1 alexa fluor 647  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology β2 ar r11e1 alexa fluor 647
    β2 Ar R11e1 Alexa Fluor 647, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 904 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B22-ar+antibodies/GR+Antibody/pm41894897-87-13-19
    Average 96 stars, based on 904 article reviews
    β2 ar r11e1 alexa fluor 647 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Sympathetic innervation maintains the murine quiescent skeletal muscle stem cell pool via perivascular-derived Angpt1.
    Article Snippet: Article Sympathetic innervation maintains the murine quiescent skeletal muscle stem cell pool via perivascular-derived Angpt1 Graphical abstract Highlights • Sympathetic nerves innervate the skeletal muscle vascular niche • Sympathetic denervation leads to muscle stem cell loss by fusion to muscle fibers • β-adrenergic signaling drives Angpt1 production in perivascular cells • Exogenous angiopoietin-1 restores the stem cell pool after sympathetic denervation Authors Alessio Rotini, Juliette Berthier, Ester Martı́nez-Sarrà, ..., Romain K. Gherardi, Peggy Lafuste, Frédéric Relaix Correspondence frederic.relaix@inserm.fr In brief Sympathetic denervation disrupts muscle stem cell quiescence by triggering premature fusion with myofibers.. Rotini et al. identify a neurovascular signaling axis involving β-adrenergic signaling and pericytederived Angpt1 as essential for stem cell maintenance and regeneration.. Rotini et al., 2025, Developmental Cell 60, 1–17 December 15, 2025 © 2025 Published by Elsevier Inc. https://doi.org/10.1016/j.devcel.2025.07.006 ll

    Article Title: CP-25 inhibits the hyperactivation of rheumatic synoviocytes by suppressing the switch in G αs -G αi coupling to the β 2 -adrenergic receptor.
    Article Snippet: .. The precleared protein was then incubated with 10 μl of Protein A/G PLUS-Agarose beads preincubated with the anti-β2AR antibody (Catalogue # sc-570, Santa Cruz, CA, USA) overnight at 4 °C with rotation. ..

    Article Title: CP-25 inhibits the hyperactivation of rheumatic synoviocytes by suppressing the switch in G αs -G αi coupling to the β 2 -adrenergic receptor.
    Article Snippet: .. Subsequently, the cells were blocked with 1% BSA for 30 min and incubated with primary antibodies, including anti-β1AR (1:600, Catalogue # sc-568, Santa Cruz Biotechnology, CA, USA), anti-β2AR (1:600, Catalogue # sc-570, Santa Cruz Biotechnology, CA, USA), anti-Gαs (1:500, Catalogue # sc-823, Santa Cruz Biotechnology, CA, USA), and anti-Gαi (1:500, Catalogue # sc-391, Santa Cruz Biotechnology, CA, USA) antibodies, overnight at 4 °C prior to 3 rinses and incubation with goat-anti-mouse Alexa Fluor 488 (1:200, Catalogue # 615–545-214, Jackson ImmunoResearch Inc., West Grove, PA, USA) or goat-anti-rabbit Alexa Fluor 555 (1:200, Catalogue # A-21428, Thermo Fisher Scientific Inc., Waltham, MA, USA) secondary antibodies for 1 h. Finally, coverslips were mounted with a mounting solution containing 4′,6-diamidino-2-phenylindole and then observed on a Leica TCS SP80 confocal microscope (Leica Microsystems, Wetzlar, Germany). β2AR coupling with Gαs and Gαi was semiquantified using the built-in colocalization analysis software module. ..

    Microscopy:

    Article Title: CP-25 inhibits the hyperactivation of rheumatic synoviocytes by suppressing the switch in G αs -G αi coupling to the β 2 -adrenergic receptor.
    Article Snippet: .. Subsequently, the cells were blocked with 1% BSA for 30 min and incubated with primary antibodies, including anti-β1AR (1:600, Catalogue # sc-568, Santa Cruz Biotechnology, CA, USA), anti-β2AR (1:600, Catalogue # sc-570, Santa Cruz Biotechnology, CA, USA), anti-Gαs (1:500, Catalogue # sc-823, Santa Cruz Biotechnology, CA, USA), and anti-Gαi (1:500, Catalogue # sc-391, Santa Cruz Biotechnology, CA, USA) antibodies, overnight at 4 °C prior to 3 rinses and incubation with goat-anti-mouse Alexa Fluor 488 (1:200, Catalogue # 615–545-214, Jackson ImmunoResearch Inc., West Grove, PA, USA) or goat-anti-rabbit Alexa Fluor 555 (1:200, Catalogue # A-21428, Thermo Fisher Scientific Inc., Waltham, MA, USA) secondary antibodies for 1 h. Finally, coverslips were mounted with a mounting solution containing 4′,6-diamidino-2-phenylindole and then observed on a Leica TCS SP80 confocal microscope (Leica Microsystems, Wetzlar, Germany). β2AR coupling with Gαs and Gαi was semiquantified using the built-in colocalization analysis software module. ..

    Software:

    Article Title: CP-25 inhibits the hyperactivation of rheumatic synoviocytes by suppressing the switch in G αs -G αi coupling to the β 2 -adrenergic receptor.
    Article Snippet: .. Subsequently, the cells were blocked with 1% BSA for 30 min and incubated with primary antibodies, including anti-β1AR (1:600, Catalogue # sc-568, Santa Cruz Biotechnology, CA, USA), anti-β2AR (1:600, Catalogue # sc-570, Santa Cruz Biotechnology, CA, USA), anti-Gαs (1:500, Catalogue # sc-823, Santa Cruz Biotechnology, CA, USA), and anti-Gαi (1:500, Catalogue # sc-391, Santa Cruz Biotechnology, CA, USA) antibodies, overnight at 4 °C prior to 3 rinses and incubation with goat-anti-mouse Alexa Fluor 488 (1:200, Catalogue # 615–545-214, Jackson ImmunoResearch Inc., West Grove, PA, USA) or goat-anti-rabbit Alexa Fluor 555 (1:200, Catalogue # A-21428, Thermo Fisher Scientific Inc., Waltham, MA, USA) secondary antibodies for 1 h. Finally, coverslips were mounted with a mounting solution containing 4′,6-diamidino-2-phenylindole and then observed on a Leica TCS SP80 confocal microscope (Leica Microsystems, Wetzlar, Germany). β2AR coupling with Gαs and Gαi was semiquantified using the built-in colocalization analysis software module. ..

    Immunofluorescence:

    Article Title: Inhibition of ezrin phosphorylation by NSC305787 attenuates procaterol-stimulated ciliary beating in airway cilia.
    Article Snippet: Ciliary beating in the airway epithelium plays an important role in preventing infection by eliminating small particles and pathogens.. Stimulation of β2 adrenergic receptor (β2AR) increases [cAMP]i levels and strongly activates this ciliary beating. β2AR is localized to the apical membrane of the airways by indirectly binding to ezrin, an actin-binding protein.. Ezrin takes active phosphorylated and inactive dephosphorylated states at Thr567.

    Western Blot:

    Article Title: Inhibition of ezrin phosphorylation by NSC305787 attenuates procaterol-stimulated ciliary beating in airway cilia.
    Article Snippet: Ciliary beating in the airway epithelium plays an important role in preventing infection by eliminating small particles and pathogens.. Stimulation of β2 adrenergic receptor (β2AR) increases [cAMP]i levels and strongly activates this ciliary beating. β2AR is localized to the apical membrane of the airways by indirectly binding to ezrin, an actin-binding protein.. Ezrin takes active phosphorylated and inactive dephosphorylated states at Thr567.



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